FAQ

  • Will Q Biotech have more application specific cartridges available?
    • Yes! We are currently working on IMAC cartridge for His-tag protein purification and a GST cartridge. Availability Q1 2025
  • I am purifying recombinant proteins from E. coli. What kind of columns can be used with the open cartridge?
    • Any column (pre-packed, such as HiTrap, or self-packed) that can be attached to the system using luer-lock connectors and can be run at low pressure (< 15 psi) can be used.  Columns without luer-lock connectors can be used with an adaptor.  For recombinant proteins from E. coli, common approach is to use His-tagged proteins.  In this case, HisTrap or Talon columns from Cytiva, HisPure column from ThermoFisher work well.  Pre-packed columns for other affinity tags are also available from these vendors.
  • Can I use low pressure columns from Cytiva and BioRad combined with the open cartridge?
    • Yes!  The columns from Cytiva, BioRad, ThermoFisher and others can be easily used with the open cartridge.  The columns often contain luer-lock adaptors for easy connection.
  • Do you have any information of the actual application cases other than IgG purification?
    • Our lab is currently working on several application notes, watch this space!
  • Are any columns compatible with the open cartridge as long as they meet with specification of the Inceptum system (Flow rate: <50 mL/min, Max pressure: 15 psi) and can be connected with the system using standard Luer-lock?
    • Correct!  Any column designed for low pressure chromatography in a water- based buffer is compatible with the Inceptum system.  Luer-lock adaptors are widely available from many vendors.
  • Are there any precautions when selecting columns for use with the open cartridges?
  • There are no precautions specific to Inceptum.  Typical recommendations for column selection include:
    • Pre-packed columns – make sure the thread to luer-lock adaptor has correct thread size.
    • Avoid organic solvents that can damage polycarbonate as the UV and conductivity flow cell contains polycarbonate.