Will Q Biotech have more application specific cartridges available?
Yes! We are currently working on IMAC cartridge for His-tag protein purification and a GST cartridge. Availability Q1 2025
I am purifying recombinant proteins from E. coli. What kind of columns can be used with the open cartridge?
Any column (pre-packed, such as HiTrap, or self-packed) that can be attached to the system using luer-lock connectors and can be run at low pressure (< 15 psi) can be used. Columns without luer-lock connectors can be used with an adaptor. For recombinant proteins from E. coli, common approach is to use His-tagged proteins. In this case, HisTrap or Talon columns from Cytiva, HisPure column from ThermoFisher work well. Pre-packed columns for other affinity tags are also available from these vendors.
Can I use low pressure columns from Cytiva and BioRad combined with the open cartridge?
Yes! The columns from Cytiva, BioRad, ThermoFisher and others can be easily used with the open cartridge. The columns often contain luer-lock adaptors for easy connection.
Do you have any information of the actual application cases other than IgG purification?
Our lab is currently working on several application notes, watch this space!
Are any columns compatible with the open cartridge as long as they meet with specification of the Inceptum system (Flow rate: <50 mL/min, Max pressure: 15 psi) and can be connected with the system using standard Luer-lock?
Correct! Any column designed for low pressure chromatography in a water- based buffer is compatible with the Inceptum system. Luer-lock adaptors are widely available from many vendors.
Are there any precautions when selecting columns for use with the open cartridges?
There are no precautions specific to Inceptum. Typical recommendations for column selection include:
Pre-packed columns – make sure the thread to luer-lock adaptor has correct thread size.
Avoid organic solvents that can damage polycarbonate as the UV and conductivity flow cell contains polycarbonate.
